Nicotinamide Riboside Malate powder is a compound of great interest in biochemical research and nutritional supplementation. Its precise analysis is critical for ensuring quality and purity. This document outlines a validated procedure for accurately determining its content using liquid chromatography. Contact us to get a more detailed MOA PDF file if interested.
English Name: 3-Carbamoyl-1-(β-D-ribofuranosyl) pyridinium malate
Molecular Formula: C₁₅H₂₀N₂O₁₀
Molecular Weight: 388.33
CAS Number: [2415659-01-5]
Analytical Method: Liquid Chromatography
The methodology follows the guidelines established in the European Pharmacopoeia (EP 2.2.29).
Chromatographic Conditions
- Column Model: Atlantis T3 (4.6 mm × 250 mm, 5 μm) or an equivalent column.
- Column Temperature: 30℃.
- Buffer Solution (pH 3.9): Prepare by dissolving 0.12 g of Ammonium Formate in 1000 mL of water suitable for chromatography. Adjust the pH to 3.9 using Formic Acid.
- Mobile Phase:
- Phase A: Buffer solution (pH 3.9).
- Phase B: Acetonitrile.
- Flow Rate: 0.8 mL/min.
- Detector Wavelength: 254 nm.
- Injection Volume: 10 μL.
Gradient Table
The gradient table specifies the variation in mobile phase composition throughout the analysis. Strict adherence to the prescribed gradient program ensures reliable results.
Preparation of Solutions
- Blank Solution: Use high-purity water.
- Test Solution:
- Weigh approximately 10 mg of Nicotinamide Riboside Malate with precision.
- Dissolve in high-purity water and dilute to 10 mL. Mix thoroughly.
- Reference Solution:
- Accurately weigh a specified quantity of the Certified Reference Standard (CRS).
- Prepare similarly to the test solution.
Analytical Procedure
- Inject the blank, test, and reference solutions sequentially into the chromatograph.
- Record the chromatograms of all solutions.
- Quantify the content of Nicotinamide Riboside Malate using the external standard method. Compare the peak areas observed in the chromatograms.
- Express the final results relative to the dried substance.
Notes and Best Practices
- Reagent Quality: The method’s reliability depends on using high-purity reagents and proper calibration of the chromatographic equipment.
- Reproducibility: Strict adherence to the described conditions, including column selection and mobile phase preparation, ensures consistent results.
- Compliance: Ensure that all results meet relevant regulatory pharmaceutical and biochemical analysis standards.
Conclusion
This comprehensive method allows for accurate quantification and rigorous quality control of Nicotinamide Riboside Malate. By following this protocol, laboratories, and analysts can support both research and industrial applications with confidence in the reliability and precision of their results.
